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mvenus integrin beta1 n 18 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc mvenus integrin beta1 n 18 plasmid
    Mvenus Integrin Beta1 N 18 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mvenus+integrin+beta1+n+18+plasmid/mVenus-Integrin-Beta1-N-18+(Plasmid+%2356330)/pm39848456-112-34-36
    Average 93 stars, based on 3 article reviews
    mvenus integrin beta1 n 18 plasmid - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: AP2A1 is upregulated upon replicative senescence of human fibroblasts to strengthen focal adhesions via integrin β1 translocation along stress fibers
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; ) and EGFP-tagged myosin regulatory light chain (MLC; ) were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen Life Technologies) following the manufacturer’s instructions with plasmids of 2.5 μ g per well of 35-mm culture dishes where cells were grown to 50–70% confluence in Opti-MEM medium.

    Article Title: AP2A1 modulates cell states between senescence and rejuvenation.
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; [45]) and EGFP-tagged myosin P. Chantachotikul et al. Cellular Signalling 127 (2025) 111616 regulatory light chain (MLC; [32]), were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen) following the manufacturer’s instructions with plasmids of 2.5 μg per well of 35- mm culture dishes where cells were grown to 50–70 % confluence in Opti-MEM medium.

    Expressing:

    Article Title: AP2A1 is upregulated upon replicative senescence of human fibroblasts to strengthen focal adhesions via integrin β1 translocation along stress fibers
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; ) and EGFP-tagged myosin regulatory light chain (MLC; ) were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen Life Technologies) following the manufacturer’s instructions with plasmids of 2.5 μ g per well of 35-mm culture dishes where cells were grown to 50–70% confluence in Opti-MEM medium.

    Article Title: AP2A1 modulates cell states between senescence and rejuvenation.
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; [45]) and EGFP-tagged myosin P. Chantachotikul et al. Cellular Signalling 127 (2025) 111616 regulatory light chain (MLC; [32]), were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen) following the manufacturer’s instructions with plasmids of 2.5 μg per well of 35- mm culture dishes where cells were grown to 50–70 % confluence in Opti-MEM medium.

    Binding Assay:

    Article Title: AP2A1 is upregulated upon replicative senescence of human fibroblasts to strengthen focal adhesions via integrin β1 translocation along stress fibers
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; ) and EGFP-tagged myosin regulatory light chain (MLC; ) were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen Life Technologies) following the manufacturer’s instructions with plasmids of 2.5 μ g per well of 35-mm culture dishes where cells were grown to 50–70% confluence in Opti-MEM medium.

    Article Title: AP2A1 modulates cell states between senescence and rejuvenation.
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; [45]) and EGFP-tagged myosin P. Chantachotikul et al. Cellular Signalling 127 (2025) 111616 regulatory light chain (MLC; [32]), were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen) following the manufacturer’s instructions with plasmids of 2.5 μg per well of 35- mm culture dishes where cells were grown to 50–70 % confluence in Opti-MEM medium.

    Generated:

    Article Title: AP2A1 is upregulated upon replicative senescence of human fibroblasts to strengthen focal adhesions via integrin β1 translocation along stress fibers
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; ) and EGFP-tagged myosin regulatory light chain (MLC; ) were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen Life Technologies) following the manufacturer’s instructions with plasmids of 2.5 μ g per well of 35-mm culture dishes where cells were grown to 50–70% confluence in Opti-MEM medium.

    Article Title: AP2A1 modulates cell states between senescence and rejuvenation.
    Article Snippet: An expression vector encoding mClover2-human AP2A1 was constructed from the pEX-A2-AP2A1 vector (Eurofins Genomics), which was digested with EcoRI and BamHI restriction enzymes and cloned into the EcoRI/BamHI-double-digested mClover2-C1 vector (Addgene plasmid#54577). .. Plasmid vectors for expressing human actin binding protein Lifeact (mRuby2-tagged Lifeact; [45]) and EGFP-tagged myosin P. Chantachotikul et al. Cellular Signalling 127 (2025) 111616 regulatory light chain (MLC; [32]), were generated as previously described. mVenus-Integrin-Beta1-N-18 plasmid (Addgene plasmid #56330) was obtained from Addgene (USA). .. Transfection was performed using Lipofectamine LTX and Plus reagent (Invitrogen) following the manufacturer’s instructions with plasmids of 2.5 μg per well of 35- mm culture dishes where cells were grown to 50–70 % confluence in Opti-MEM medium.



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